Fig. 5: Inhibition of neddylation in CA1 impairs object location memory and the learning-induced degradation of DNMT3A1. | Neuropsychopharmacology

Fig. 5: Inhibition of neddylation in CA1 impairs object location memory and the learning-induced degradation of DNMT3A1.

From: Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus

Fig. 5

a Schematic representation of the experimental protocol for object location memory. b, c DNMT3A1 protein levels are reduced 3 h after training. Unpaired Student’s t-test *p < 0.05. d, e DNMT3A1 protein levels are not changed in trained mice 6 h after training. Histone was used as a loading control for normalization. Unpaired Student’s t-test n.s. = not significant. f Injection timeline and representative image of injection site in CA1. g Intra-hippocampal infusion of MLN4924 reduces the discrimination index of animals tested in the object location memory test. Unpaired Student’s t-test with Welch correction ***p < 0.001. h, i Learning induced reduction of DNMT3A1 protein levels in trained, but not MLN4924 injected mice 3 h after training. Unpaired two-tailed Student’s t-test *p < 0.01. j, k DNMT3A1 protein levels were not altered in trained MLN4924 injected or control mice 6 h after training. Histone was used as a loading control for normalization. Data are represented as mean ± S.E.M. Unpaired Student’s t-test *p < 0.05. Sample numbers for each experimental group indicate mice used per group.

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