Table 1 Effect of 50 nM CRF on intrinsic properties of insular cortex pyramidal neurons.

From: Insular cortex corticotropin-releasing factor integrates stress signaling with social affective behavior

Parameter

Sex

Means (SEM)

Results

Summary

aCSF

CRF

Main effects

Interaction

Membrane potential (mV)

M

−66.65 (1.527)

−59.64 (2.007)

CRF, F1,24 = 72.93, P < 0.0001

F1,24 = 5.124, P = 0.033

⇧

F

−68.41 (1.189)

−64.33 (1.268)

Sex, F1,24 = 2.267, P = 0.145

 

⇧

Rheobase (pA)

M

1256.42 (105.92)

1226.42 (129.94)

CRF, F1,24 = 0.282, P = 0.600

F1,24 = 0.924, P = 0.346

=

F

1515.00 (132.76)

1619.17 (157.49)

Sex, F1,24 = 3.573, P = 0.070

 

=

Spike amplitude (mV)

M

73.35 (3.852)

70.99 (4.039)

CRF, F1,24 = 4.933, P = 0.036

F1,24 = 0.263, P = 0.612

⇩

F

80.58 (3.764)

76.80 (4.136)

Sex, F1,24 = 1.430, P = 0.243

 

⇩

AP rise rate (mV/ms)

M

181.1 (17.55)

157.6 (14.548)

CRF, F1,24 = 18.93, P = 0.0002

F1,24 = 0.535, P = 0 .472

⇩

F

229.2 (25.536)

196.2 (26.136)

Sex, F1,24 = 2.253, P = 0.146

 

⇩

AP half-width (ms)

M

1.248 (0.075)

1.357 (0.064)

CRF, F1,24 = 16.69, P = 0.0004

F1,24 = 0.134, P = 0.717

⇩

F

1.125 (0.074)

1.256 (0.098)

Sex, F1,24 = 1.121, P = 0.300

 

⇩

After depolarization (mV)

M

10.36 (1.365)

8.593 (1.312)

CRF, F1,24 = 26.83, P < 0.0001

F1,24 = 2.142, P = 0.156

⇩

F

12.98 (0.805)

9.808 (0.688)

Sex, F1,24 = 1.554, P = 0.225

 

⇩

Burst ratio

M

0.2608 (0.052)

0.3889 (0.055)

CRF, F1,21 = 38.82, P < 0.0001

F1,21 = 3.769, P = 0.066

⇧

F

0.2153 (0.032)

0.2825 (0.024)

Sex, F1,21 = 1.600, P = 0.220

 

⇧

Input resistance (MΩ)

M

130.9 (15.318)

120.9 (14.663)

CRF, F1,24 = 5.985, P = 0.0221

F1,24 = 2.497, P = 0.127

⇩

F

104.9 (13.460)

102.8 (14.249)

Sex, F1,24 = 1.156, P = 0.293

 

=

Time constant (ms)

M

21.71 (0.946)

20.07 (0.946)

CRF, F1,24 = 12.37, P = 0.0018

F1,24 = 0.173, P = 0.681

=

F

24.25 (0.799)

22.17 (0.726)

Sex, F1,24 = 4.235, P = 0.051

 

⇩

Rectification ratio (mV)

M

0.8343 (0.023)

0.9014 (0.023)

CRF, F1,24 = 35.28, P < 0.0001

F1,24 = 0.611, P = 0.442

⇧

F

0.8158 (0.032)

0.9033 (0.025)

Sex, F1,24  = 0.058, P = 0.810

 

⇧

Sag ratio

M

1.359 (0.175)

1.384 (0.181)

CRF, F1,24  = 1.339, P = 0.259

F1,24 = 2.072, P = 0.163

=

F

1.555 (0.244)

1.331 (0.231)

Sex, F1,24 = 0.065, P = 0.801

 

=

sAHP (mV)

M

−655.21 (85.958)

−765.43 (84.764)

CRF, F1,24 = 3.080, P = 0.092

F1,24 = 0.517, P = 0.479

=

F

−659.41 (49.605)

−705.58 (44.492)

Sex, F1,24 = 0.092, P = 0.479

 

=

Firing rate (spikes/s @250 pA)

M

57.214 (17.354)

51.143 (19.000)

aCRF, F1,24 = 9.611, P = 0.005

aF1,24 = 0.440, P = 0.503

⇩

F

52.092 (16.433)

38.050 (14.649)

aSex, F1,24 = 0.010, P = 0.921

 

=

  1. Data were analyzed with a two-way analysis of variance. Summary indicates the effect of CRF and sex on parameter measured. Arrows indicate significant differences between aCSF and CRF conditions (Ps < 0.05, Sidak-corrected t-test).
  2. aThe distribution of firing rates were negatively skewed so the data were converted with a log transform prior to analysis with ANOVA.
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