Fig. 1: Sub-sedative AlloP acutely alters mid frequency range oscillations in active wake EEG.
From: Non-sedative cortical EEG signatures of allopregnanolone and functional comparators
A Representative spectrograms showing time course of acute drug exposure including baseline, vehicle, and drug periods. Black lines indicate vehicle and drug injection, horizontal white lines indicate active segments used for further analysis, and white overlay represents animal activity measured via accelerometer. B, C AlloP effects on frontal (B) and parietal (C) active wake EEG oscillations, represented as the proportion of analyzed time segment that an oscillation at a given frequency was present (Pepisode; see Methods). Repeated measures two-way ANOVA showed an interaction between drug treatment X oscillation frequency at both electrodes analyzed (Frontal – F(198,800) = 41.14, p < 0.0001, Parietal – F(198,800) = 33.59, p < 0.0001). Horizontal bars indicate difference from vehicle after Dunnett’s multiple comparisons testing (black – baseline, orange – vehicle, red – AlloP (5 mg/kg)). D Peak Pepisode of theta oscillations in parietal EEG. Right panel shows 95% confidence interval of mean difference of Pepisode estimate. One-way repeated measures ANOVA revealed an effect of treatment on Pepisode (F(1.498, 5.991) = 49.38 p = 0.0003). Dunnet’s multiple comparison showed a difference between Vehicle (VEH) and AlloP (Drug, D) peak (p = 0.0045). Baseline (no injection) is denoted as BL. E Integrated bandpower of vehicle-normalized Pepisode distribution from frontal electrode. Dotted line indicates vehicle (normalizing condition). Black symbols denote the baseline condition. Red symbols denote AlloP. Two-way repeated measures ANOVA showed drug treatment X frequency band interaction (F(4, 20) = 52.20 p < 0.0001). Dunnett’s multiple comparisons showed difference between vehicle and AlloP for alpha (p = 0.0075), beta (p < 0.0001) and gamma (p < 0.0001) band power. F Integrated band power of vehicle-normalized Pepisode distribution from parietal electrode. Two-way repeated measures ANOVA revealed drug treatment X frequency band interaction (F(4, 20) = 37.41 p < 0.0001). Dunnett’s multiple comparisons showed difference between baseline and AlloP for alpha (p = 0.0003), beta (p = 0.0241) and gamma (p < 0.0001) band power. Black circles and bars to the right of treatment groups in (E, F) represent estimate of mean difference between drug and baseline treatment ± 95% confidence intervals calculated from Dunnett’s multiple comparisons test.
