Fig. 2: Pentobarbital and diazepam share features with AlloP on EEG.

Representative spectrograms showing time course of acute effects of pentobarbital (A) and diazepam (D) on frontal (top panels) and parietal (bottom panels) EEG. Black lines indicate vehicle and drug injection, horizontal white lines indicate active segments used for further analysis, and white overlay is trace from head-mounted accelerometer. B Pentobarbital effects on frontal (left panel) and parietal (right panel) active wake EEG oscillations. Two-way ANOVA showed frequency X drug interaction in both frontal (F(198, 1200) = 39.29, p < 0.0001) and parietal (F(198, 1200) = 51.17, p < 0.0001) electrodes. C Parietal theta rhythm peak was reduced (one-way ANOVA, F(1.204, 7.227) = 32.89, p = 0.0001). Dunnett’s multiple comparisons test between vehicle and pentobarbital revealed a difference (p = 0.0005). E Diazepam induced changes in frontal (left panel) and parietal (right panel) active wake EEG oscillations. Two-way ANOVA showed frequency X drug interaction for frontal (F(198, 800) = 30.72, p < 0.0001) and parietal (F(198, 800) = 31.48, p < 0.0001) electrodes. F Theta frequency peak was reduced during acute diazepam effects (One-way ANOVA, effect of drug treatment on peak F(1.218, 4.870) = 47.24, p = 0.0009). Dunnett’s multiple comparisons showed difference between vehicle and diazepam (p = 0.0072). Horizontal lines in B, E represent difference from vehicle spectra following Dunnett’s multiple comparisons test with an alpha threshold of 0.05.