Fig. 5: Impaired response inhibition during activation of GABAergic BF input to the LHb by ChR2 in GADCre rats.
From: Basal forebrain-lateral habenula inputs and control of impulsive behavior
A Comparison of the percent of neutral stimulus (NS) trials in which responses on the active lever were observed in the absence (off) and presence of 455 nm light delivered via optical fibers terminating above the LHb, ~8 weeks after expression of Cre-dependent eYFP in the VP/NAcs (n = 6 rats; paired t test, t5 = 1.67, p = 0.155). B Optogenetic inhibition of VP/NAcs GABAergic inputs to LHb by stimulation of NpHR with 545 nm light does not affect NS responding (n = 4 rats; paired t test, t3 = 0.38, p = 0.728). C Activation of VP/NAcs GABAergic inputs by light stimulation of ChR2 in LHb significantly increased the percent of NS trials in which responses were observed (paired t test; n = 10 male rats, t9 = 3.44, p = 0.007). The total number of NS responses shown in Fig. S4A. D ChR2 photostimulation with 455 nm light significantly increases responding during presentation of the NS, and this is not significantly different between male and female GADCre rats (n = 16 male and 11 female rats; 2-way RM ANOVA, light x sex interaction, F1,25 = 1.546, p = 0.225; main effect of light, F1,25 = 41.03, p < 0.0001; Post hoc comparisons by uFLSD, effect of light in males, p < 0.0001; effect of light in females, p = 0.0025). ns, non-significant.
