Fig. 2: Verification of the KO line and characterization of body weight, adrenal weight and CORT levels. | Neuropsychopharmacology

Fig. 2: Verification of the KO line and characterization of body weight, adrenal weight and CORT levels.

From: Deep phenotyping reveals CRH and FKBP51-dependent behavioral profiles following chronic social stress exposure in male mice

Fig. 2

A The representative images are taken from the paraventricular nucleus (PVN) region of the mouse brain with a magnification of 20x confocal microscope objective. B The quantification points in the graphs represent Fkbp5 expression in Crh positive cells from pooled regions with CRH expression: dHipp, ceAmy, BNST, PVN, and PFC. A significant reduction of raw count of double positive cells (Crh and Fkbp5) was observed between the KO-line (p < 0.001) and wt (p < 0.001). This reduction was notable in both control and CSDS exposed animals. (stress effect: #=p < 0.05, ##=p < 0.01, ###=p < 0.001, ####=p < 0.0001, genotype effect: *=p < 0.05). C The specificity of the KO has been validated by counting the positive puncta of Fkbp5 in Crh negative cells from the aforementioned regions. With significantly elevated Fkbp5 levels in the CSDS condition (p < 0.0001), but no differences between the two genotype groups. D Baseline corticosterone (CORT) levels marked no differences between stress conditions nor genotype. E The body weight of the animals marked an overall increase over the experimental timeline. There was a main effect of genotype, without post-hoc tests reaching significant differences. F The adrenal weight of CSDS animals was significantly elevated (p < 0.01) but no effect of genotype was marked.

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