Fig. 5: Overnight behavioral classifiers analysis provides an insight into longitudinal interaction between the CD1 and experimental animals in a home-cage-like environment.
A Illustration of the experimental set-up of the overnight-recording paradigm, for either CD1 and experimental animal or two littermates. In both cases, divided in the middle with perforated plexiglass. B No significant changes in velocity were detected over the 12 hours between the groups. C Comparison of distance travelled between the genotypes of control group. Significant difference was observed during 9th hour of recording (p < 0.05) (stress effect: #=p < 0.05, ##=p < 0.01, ###=p < 0.001, ####=p < 0.0001, genotype effect: *=p < 0.05). D Comparison of distance travelled between the genotypes within the CSDS group, the deviating patterns did not reach significance level. E–J Behavioral classifiers of the first 0–3 h of recording. We can observe significant upregulation of looking around behavior (p < 0.01) together with nose to nose interaction (p < 0.01) and downregulation of climbing (p < 0.05) in the CSDS group in comparison to the controls. K The exploratory index over this period showed to be similar across all the groups. L The social score illustrates an increase in social activity of the CSDS group, irrespective of genotype. (M-R) The analysis of behavior from the time window 7–10 h showed preservation of upregulated looking around behavior (p < 0.01) and nose to nose interaction (p < 0.01). Additional parameters were marked as significant during this time, being sniffing behavior (p < 0.05) and nose to body (p < 0.05). S The exploratory index from 7 to 10 h period showed this time a significant effect only in the CSDS group, where CRHfkbp5−/− animals showed a significant decreased exploration in comparison to their wt counterparts. T The social score remained to be significantly upregulated also at this later stage in the observation period (p < 0.05).
