Fig. 5: Identification of inputs driving the difference in connectivity to VTADA cells following adolescent THC exposure.
From: Neural basis of adolescent THC-induced potentiation of opioid responses later in life
A Schematic and explanation for how PCA analysis was carried out on the RABV tracing dataset. B Proportion of cumulative variance explained by each principal component. C Plot of PC1 and PC2 from brains of control and THC-treated mice. D Plot of PC1 and PC3 from brains of control and THC-treated mice. E Heatmap of the contributions of each brain region, or feature, in the data to PCs 1 through 3. Comparison of total PC values for each brain along (F) PC 1, (G) PC 2, and (H) PC 3. F Control 3.3 vs. THC −2.2, p = 0.0002. G Control 0.13 vs. THC −0.09, p = 0.89. H Control 0.57 vs. THC −0.38, p = 0.48. I To further quantify relationships between brains and conditions, the Euclidean distance between each brain from control and THC-treated mice in the PC1 versus PC2 coordinate space was calculated. These distances were then plotted in a heatmap with brains being organized by similarity assessed by hierarchical clustering.
