Fig. 1: Derivation of PBMC microglia-like cells (piMGLCs). | Neuropsychopharmacology

Fig. 1: Derivation of PBMC microglia-like cells (piMGLCs).

From: Identifying brain-penetrant small-molecule modulators of human microglia using a cellular model of synaptic pruning

Fig. 1

A Representative phase-contrast image of piMGLCs. Scale bar, 100 μm. B Confocal images of piMGLCs stained for indicated canonical microglial markers IBA1, PU.1, CX3CR1, and P2RY12. Scale bar, 100 μm. Image-based phagocytosis assay optimization for CNS-penetrant compound HTS screening. C Overview workflow for large-scale batch derivation of piMGLCs and 96-well functional synaptosome phagocytosis image-based screens. D Z’ factor = 0.51 was determined using 0.1% DMSO (green) as negative control and 2 μM cytochalasin D (red) as positive control phagocytosis inhibitor, 24 wells of each. Data points represent phagocytic indices of each well, calculated as the sum of synaptosome area divided by the sum of cell count within 12 image fields per well. Black dashed lines are the group means, and colored dashed lines signify one standard deviation around the mean for the group of the corresponding color.

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