Fig. 5: MDD and suicide-associated m6A RNA methylation profiling and functional annotation in the dlPFC following microarray analysis.

A Expression heatmap displaying normalized methylation enrichment values across samples, comparing MDD non-suicide (MDD-NS; n = 32) and MDD suicide (MDD-S; n = 17) subjects. A cluster dendrogram was generated using 100 differentially methylated transcripts based on hierarchical clustering. B Volcano plot from m6A microarray methylation analysis showing differentially methylated transcripts between MDD-NS and MDD-S groups. Red and blue dots indicate hypo- and hypermethylated transcripts, respectively. C Stacked bar diagram showing distribution of hyper- and hypomethylated transcripts across MDD-NS, MDD-S, and combined MDD (MDD-NS + MDD-S) groups. D Venn diagram illustrating overlap of significantly hypermethylated transcripts (FC ≥ 2) between Control vs MDD and Control vs MDD-NS comparisons. A total of 33 genes were unique to MDD, 196 exclusive to MDD-NS, and 22 shared. E Venn diagram showing transcript overlap between Control vs MDD and Control vs MDD-S groups, with 17 genes unique to MDD, 1,148 unique to MDD-S, and 38 shared. F GO enrichment network highlighting significantly affected functional terms based on inter-node betweenness and edge strength. G Ridgeline plots displaying enrichment of neuronal functional categories linked to hypermethylated coding genes in MDD-S. H PPi network showing direct and indirect associations among hypermethylated transcripts in MDD-S, derived from STRING database criteria.