Fig. 1: Multi-omics analysis integrating TF-activity profiling and transcriptomic data in stressed mice.
From: Transcription factors Lef1 and Rest stimulate recovery from depressive states
a Schematic illustration of TF-activity profiling in vivo. The brain image shows the reporter infected cells (red, flag-tag) in the prefrontal cortex. Scale bar, 500 μm. b Scheme of repeated social defeat. c–e Effects of chronic social defeat stress on behaviors. c The result of the SI-test for control (black), resilient (yellow), and susceptible (red), Welch’s t-test, n = 71, 119. d The result of the open field test. Tukey’s test; control, n = 34; resilient, n = 26; susceptible, n = 20 mice. e The result of the sucrose preference test. Tuley’s test; control, n = 28; resilient, n = 36; susceptible, n = 20 mice. f Radar graph showing changes in TF-activities in susceptible mice, normalized to levels in control mice. For each TF, the corrected mean ± sem of TF-activities are shown as log2-fold changes. See Supplemental Methods for details. g Heatmap illustrating the scaled transcripts per million (TPM) values of DEGs in the susceptible mice, compared to the same genes in control mice. RNA-seq analysis was performed on 3 mice from both the control and susceptible groups. h Scatter plot showing alterations in the transcriptional activity of each TF and the average change in expression of its downstream genes.
