Fig. 1: Adolescent repeated ethanol exposure (AIE) induces anxiety-like behaviors in adult mice and increased neuronal activities in the PVT. | Neuropsychopharmacology

Fig. 1: Adolescent repeated ethanol exposure (AIE) induces anxiety-like behaviors in adult mice and increased neuronal activities in the PVT.

From: Glutamatergic dysfunction of astrocytes in paraventricular nucleus of thalamus contributes to adult anxiety susceptibility in adolescent ethanol exposed mice

Fig. 1

Diagram to explain the experimental procedure (a). Representative traces (b, e) and pooled data (c, d, f, g) showing that adult mice at 4 weeks withdrawal from repeated ethanol exposure during adolescent period (AIE) show heightened anxiety-like behaviors compared to air-exposed counterpart mice (CON) in open field test (b–d) and elevated plus maze test (e–g). Diagram and representative traces (h, i) and pooled data (j) showing that increased spontaneous firing of PVT neurons after AIE. Representative expression of GCaMP6s in the PVT (k), behavior-synced calcium traces (l), and pooled data (m) showing that the calcium transients in the PVT neurons are significantly increased in the AIE group when exposed anxiogenic behavioral task. Female (circles) and male (triangles). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Fig. 1a, b, e, h, and k were created using BioRender.com.

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