Fig. 2: Chemogenetic inhibition of PVT neurons alleviates anxiety-like behaviors induced by AIE. | Neuropsychopharmacology

Fig. 2: Chemogenetic inhibition of PVT neurons alleviates anxiety-like behaviors induced by AIE.

From: Glutamatergic dysfunction of astrocytes in paraventricular nucleus of thalamus contributes to adult anxiety susceptibility in adolescent ethanol exposed mice

Fig. 2

a–c Diagram of experimental schedules (a) and representative figures showing the expression of hM4Di-mCherry in the PVT. (c) Representative traces showing the silence of neuronal activity in the hM4Di-positive neurons in the PVT by bath application of DREADDs ligand (J60, 20  \(\mu\)M). Represenetative traces (d, g) and pooled data (e, f, h, i) showing that the inhibition of PVT neuronal activities by chemogenetic application rescues the anxiety-like behaviors seen in AIE mice in the open filed test (d–f) and elevated plus maze test (g–i). Female (circles) and male (triangles). Data represented as mean ± SEM. **p < 0.001, ***p < 0.0001. J60: JHU37160 (0.3 mg/kg, i.p.). Fig. 2a, b, d, and 2g were created using BioRender.com.

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