Fig. 4: Region-specific conditional knock-out of GLT1 in the PVT mimics the anxiety-like behaviors induced by adolescent ethanol exposure. | Neuropsychopharmacology

Fig. 4: Region-specific conditional knock-out of GLT1 in the PVT mimics the anxiety-like behaviors induced by adolescent ethanol exposure.

From: Glutamatergic dysfunction of astrocytes in paraventricular nucleus of thalamus contributes to adult anxiety susceptibility in adolescent ethanol exposed mice

Fig. 4

a Schematic drawing of Cre-loxP system for GLT1 cKO and genotyping of GLT1 wild-type and flox mouse. The representative figures (b) and pooled data (c) showing the reduction of GLT1 levels in the PVT after the GFAP-promoter driven expression of Cre in the PVT of GLT1 flox mouse. Representative traces (d) and pooled data (e) showing that the DHK-induced changes in neuronal firing were occluded in the PVT of GLT1 cKO. Representative traces (f, i) and pooled data (g, h, j, k) showing the effects of GLT1 cKO in the PVT astrocytes in the open field test (g, h) and elevated plus maze test (i–k). Female (circles) and male (triangles). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001. Fig. 4a, f, and i were created using BioRender.com.

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