Fig. 7: Melatonin attenuates adipose tissue and liver inflammation in HFD-fed mice.

A F4/80 immunostaining of eWAT. Scale bars = 100 μm. B Levels of pro- and anti-inflammatory cytokine mRNAs in eWAT. C F4/80 immunostaining of the liver. Scale bars = 100 μm. D Levels of F4/80, and pro- and anti-inflammatory cytokine mRNAs, in the liver. E Immunoblots of phosphorylated p38 MAPK and eIF2α, and their total protein contents in the liver. Data are mean ± SEM, n = 5–8. Significance was determined by one-way ANOVA. *p < 0.05, **p < 0.01 vs. NC + Veh mice; #p < 0.05, ##p < 0.01, vs. HFD + Veh mice.