Fig. 1: Comparison of visceral adipose tissue DNA methylation profiles between patients with obesity but without diabetes (OND) and patients with obesity and diabetes (OD). | Nutrition & Diabetes

Fig. 1: Comparison of visceral adipose tissue DNA methylation profiles between patients with obesity but without diabetes (OND) and patients with obesity and diabetes (OD).

From: DNA methylation and gene expression analysis in adipose tissue to identify new loci associated with T2D development in obesity

Fig. 1

a Volcano plot shows differences in methylation. Points represent all analyzed CpGs, and blue points indicate DMCs (FDR < 0.05): lighter blue hypomethylated and darker blue hypermethylated in OD, and gray points non-significant. b Heat map of DMCs showing DNA methylation levels for each CpG (row) by patient (columns), after applying an unsupervised hierarchical clustering analysis. c Distribution of the DMCs among the different genomic locations. d Distributions of DMCs across the CGI, the shore (2 kb from the CGI), the shelf (2–4 kb from the CGI), and the open sea (the remaining genome) regions. e Gene set enrichment analysis of DMCs (Affinity propagation). Kyoto Encyclopedia of Genes and Genomes pathway enrichments associated with genes with DMCs. X axis: FDR (false discovery rate) value. DMCs differentially methylated CpGs, TSS1500 1500–200 bp upstream of the transcriptional start site (TSS), TSS200 200 bp upstream of the TSS, IGR intergenic regions, UTR untranslated region, CGI CpG island.

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