Table 1 Primer sequences of genes for quantitative real-time PCR.
From: Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes
Gene | Primer sequence |
|---|---|
Sirt1 | forward, 5’-ATGACGCTGTGGCAGATTGTT-3’ |
|  | reverse, 5’-CCGCAAGGCGAGCATAGAT-3’ |
Sirt3 | forward, 5’-CTACATGCACGGTCTGTCGAA-3’ |
|  | reverse, 5’-GCCAAAGCGAAGTCAGCCATA-3’ |
Drp1 | forward, 5’-CAGGTGGTGGGATTGGAGAC-3’ |
|  | reverse, 5’-CTGGCATAATTGGAATTGGTTT-3’ |
Opa1 | forward, 5’-CCGAGGATAGCTTGAGGGTT-3’ |
|  | reverse, 5’-CGTTCTTGGTTTCGTTGTGA-3’ |
Mfn2 | forward, 5’-TTCTTGTGGTCGGAGGAGTG-3’ |
|  | reverse, 5’-CTTTGGTGGTCCAGGTCAGT-3’ |
Sod1 | forward, 5’-AACCAGTTGTGTTGTCAGGAC-3’ |
|  | reverse, 5’-CCACCATGTTTCTTAGAGTGAGG-3’ |
actin | forward, 5’-TTCAACACCCCAGCCATG-3’ |
|  | reverse, 5’-CCTCGTAGATGGGCACAGT-3’ |
