Fig. 5: Non-alcoholic fatty liver disease (NAFLD) in diabetic db/db mice.

A Schematic illustration of the experimental procedure. db/m and db/db mice were fed normal diet until the age of 8 months (M). B Images comparing db/m and db/db mice. Blue arrows indicate db/m mice, red arrows indicate db/db mice. C Body weight of db/m and db/db mice. D Blood glucose analysis of db/m and db/db mice. E Images comparing the livers of db/m and db/db mice. F Liver weight of db/m and db/db mice. Statistical analyses in C, D, and F were performed using Student’s t-test. *P < 0.05; ***P < 0.001. G H&E staining of liver sections from db/m and db/db mice. Scale bars, 100 μm (upper panels) and 50 μm (lower panels). Each dot represents one mouse; n ≥ 3 in each group.