Fig. 5
From: Activated ALK signals through the ERK–ETV5–RET pathway to drive neuroblastoma oncogenesis
RET is a target of the transcription factor ETV5. a Western blotting of ETV5 and RET after ETV5 invalidation using 3 different siRNA in SK-N-BE(2)C cells at 48 h. Actin is used as a loading control. siCT: control. b The levels of ETV5 and RET mRNA were measured after ETV5 invalidation at the same time point and compared to the expression levels in the siCT condition. c Luciferase reporter assay for the RET promoter region. Luciferase activity was evaluated upon ETV5 expression from a pcDNA3-ETV5 plasmid used at two quantities in HEK cells and normalized to empty pcDNA3. Results are shown for the empty reporter vector and reporter vector bearing the RET promoter. d Matrix of the ETV5 transcription factor defined by ChIP-Seq. e ETV5-binding peaks revealed by ChIP-seq analysis. Binding regions defined by HMCan are shown in colors. Regions in blue show peaks overlapping the RET promoter, whereas region in green indicates a strong peak likely acting as an enhancer
