Fig. 4

The transcriptional regulon of ASCL1, E2F7, NFIA and SMAD2. a A representative regulatory network using iRegulon in Cytoscape. The large nodes represent the genes regulated by E2F7, NFIA, SMAD2 and ASCL1. The target genes for each TF are portrayed in red if they are up regulated with SRC-1 or dark blue if they are down regulated with SRC-1 and light blue, yellow or orange for pathways in cancer, cell differentiation or cell cycle respectively. The shared targets between the 4 TFs are CDKN1A and CENPF. b From the regulatory network, 79 genes were found to be regulated by two or more of the SRC-1 regulated TFs. The expression of these genes was profiled in primary breast tumours of patients that responded well to therapy (n = 3) and in matched primary (n = 3) vs. local recurrence samples (n = 3) from patients who had disease recurrence on endocrine treatment (from left to right). c Functional annotation of a subset of these genes in primary tumours from patients that recurred on endocrine treatment and their matched local recurrence. ‘*’ denotes those genes highlighted in the text