Fig. 4

miR-155 promotes glucose metabolism through activation of p85α-FOXO3a-cMYC in vivo. a Growth curve of allografts (n = 6 for each group). miR-155^+/ko cells (Lav670, red; Lav714, in Blue) or paired miRZIP155 cells (Lav670 miRZIP155, in green; Lav714 miRZIP155, in orange) were allografted and tumor growth was measured. b Pictures showing the size measurement of xenograft tumors (n = 5 for each group) that were generated in the panel (a). c Western blot images showing the level of GLUT1, HK2, PKM2, LDHA, cMYC, p85α and FOXO3a in allograft tumors. d–j Graphs showing the densitometry results of the panel (c). Normalized band intensity of GLUT1 (d), HK2 (e), PKM2 (f), LDHA (g), FOXO3a (h), p85α (i), and cMYC (j) are shown. k Flow diagram showing the results of LC-MS/MS analysis measuring glucose and its metabolite from miR-155^ko/+ (in black) or paired miRZIP155 cells (in blue). The left panel shows data from Lav714 and the right panel is from Lav 670. l–o Graphs showing the level of glucose and its metabolites as well as NAD measured from the control (in blue) or miRZIp155 (in red) allograft tumors. Relative levels of glucose (l), glucose-6-phosphate (m), pyruvate (n), NAD (o) are shown