Fig. 7
Negative feedback loop of Wnt/β-catenin signaling through upregulation of C9orf140. a The 293T cells were treated with Wnt3A-CM and harvested at the indicated times. Cell lysates were subjected to western blot analysis for the indicated proteins. The right panel shows quantitative analysis of β-catenin and C9orf140 levels normalized to GAPDH using Image Lab software (Bio-Rad). Data are presented as average fold induction relative to the control ± SD. b The 293T cells were first transfected with 20 nM negative control or β-catenin siRNA and then treated with Wnt3A-CM for 18 h. Cells were harvested 48 h after transfection and lysed for western blot analysis (upper panel). The lower panel shows quantitative analysis of C9orf140 levels normalized to GAPDH. c A model depicting the proposed mechanism for negative feedback regulation of Wnt/β-catenin signaling by C9orf140
