Fig. 7 | Oncogene

Fig. 7

From: Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10

Fig. 7

MiR-23a directly targets TSGA10. a Schematic of predicted miR-23a binding sequence in TSGA10-3′-UTR. b Representative IHC images of TSGA10 in tissues collected from graph-depicted groups. TSGA10 staining was mainly localized in the cytoplasm of cells. c Overexpression of miR-23a reduced TSGA10-3′-UTR luciferase activity in vitro but not mutated TSGA10-3′-UTR luciferase activity. Student’s t-test. d–g TSGA10 immunoblotting in HUVECs treated as indicated. The results were analyzed with ImageJ software. One-way ANOVA. h, i Interference efficiency was detected by western blot. One-way ANOVA. j, k Transwell migration assays were performed to measure cell migration. One-way ANOVA. l, m HUVECs treated as indicated and analyzed by western blot. Student’s t-test. n, o Transwell migration assays were performed to measure cell migration. One-way ANOVA. p–r HUVECs treated as indicated were analyzed by western blot. One-way ANOVA. s Transwell migration assays were performed to measure cell migration. One-way ANOVA

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