Fig. 1
Copy number gain of DDX27 contributed to mRNA overexpression in human CRC. a Data of CNA across 24776 genes of 615 TCGA CRC samples. Gene-level frequency of copy number gain and loss was calculated and ranked. Percentage of samples (y axis label of the graph) of individual gene was determined by subtracting frequency of copy number loss from frequency of copy number gain. List of top ranked genes (n = 23) with the highest frequency of copy number gain was shown in Table S1. b CNAs of DDX27 in 103 primary CRC tissues from Beijing cohort. c Comparison of DDX27 mRNA expression (left panel) and protein expression (right panel) between CRC without DDX27 CNA (wt) and CRC with DDX27-amplified (amp) in Beijing cohort. Middle panel showed the correlation between DNA copy number and mRNA expression of DDX27. d Left panel showed comparison of DDX27 mRNA expression between CRC without DDX27 CNA (wt) and CRC with DDX27-amplified (amp) in TCGA cohort. Right panel showed the correlation between DNA copy number and mRNA expression of DDX27 in CRC. e The mRNA expression of DDX27 in 151 pairs of CRC (T) and adjacent normal tissue (N) (left panel), and protein expression of DDX27 in 6 pairs of primary CRC (T) and adjacent normal tissue (N) (right panel) from Beijing cohort were detected. f Kaplan–Meier survival analysis indicated that CRC patients with high expression of DDX27 had worse relapse-free survival in Beijing cohort. g Left panel showed differential expression of DDX27 mRNA between colon cancer and rectal cancer in Beijing cohort. Kaplan-Meier survival analysis of DDX27 in predicting relapse-free survival for patients with colon cancer (middle panel) or rectal cancer (right panel) was exhibited. h Representative images of DDX27 protein expression in CRC tumor tissues (T) and adjacent normal tissue (N) of three patients (P1-P3) from Shanghai cohorts by immunohistochemistry. Staining score of DDX27 was quantified accordingly. i Stratification of the patients in Shanghai cohort according to DDX27 staining score showed that high DDX27 expression (score ≥ 8) predicted poor overall survival (right panel). (*P < 0.05; **P < 0.01; ***P < 0.001)
