Fig. 4
DDX27 promoted metastatic ability of CRC cells. a Representative images of wound-healing assay indicated that ectopic expression of DDX27 promoted cell migration in HCT116 and SW480. b Representative images of matrigel invasion assay revealed that ectopic expression of DDX27 promoted CRC cells invasion. c Western blot analysis revealed that ectopic expression of DDX27 increased expression of mesenchymal markers (Slug and Vimentin) while decreased expression of epithelial markers (E-cadherin). d Representative images of matrigel invasion assay revealed that silencing of DDX27 reduced CRC cells invasion. e DDX27 silencing decreased expression of mesenchymal markers (Slug and Vimentin) while increased expression of epithelial markers (E-cadherin) as indicated by Western blot analysis. f Representative macroscopic appearances of lung metastasis are shown (tumor nodules were indicated as black arrows in top panel). Representative hematoxylin-eosin stained images of the lungs were shown in the bottom panel. Quantification of lung metastatic lesions in HCT116-shCTL (N = 6), HCT116-shDDX27-1 (N = 7) and HCT116-shDDX27-2 (N = 7) groups. Data was expressed as mean ± S.E.M. Student’s t-test was performed. (*P < 0.05; **P < 0.01; ***P < 0.001)
