Fig. 5
From: NGR (Asn-Gly-Arg)-targeted delivery of coagulase to tumor vasculature arrests cancer cell growth
Therapeutic potential of tCoa-NGR proteins in vivo. a Illustrative photos of mice bearing prostate cancer xenografts (PC3) at the end of treatment (day 7) treated with tCoa-NGR (right) or tCoa (left). b 4T1 and PC3 tumor-bearing mice were injected intravenously with saline, 10 µg tCoa or 10 µg tCoa-NGR (n = 6). c Histological analysis of 4T1 mouse mammary xenografts in BALB/c mice and PC3 human prostate carcinoma xenografts in C57Bl/6 nude mice treated with either saline, tCoa or tCoa-NGR fusion proteins. Arrows represent intact vessels throughout tumor tissue sections in mice treated with saline. Conversely, tCoa-NGR tumor tissues presented occluded blood vessels with packed erythrocytes and fibrin clots, indicating an induction of thrombosis in the vasculature of 4T1 and PC3 tumors. Several thrombosed blood vessels are selected, and the same location is shown with two different magnifications for clarity. Moreover, induction of complete thrombosis in the neovasculature of mice bearing 4T1 or PC3 tumors was explored through distinct staining of fibrin (red) and red blood cells (yellow) by Masson’s trichrome staining
