Fig. 1
miR-483-3p regulates gefitinib sensitivity in EGFR-mutant lung cancer in vitro and in vivo. a, b Quantitative RT-PCR analysis of hsa-miR-483-3p levels in PC9GR compared with PC9 a and in HCC827GR compared with HCC827 b. RNU6B is used for normalization. c, d HCC827 xenograft tumors in immunocompromised mice were treated with gefitinib (25 mg/kg/day) orally until tumor regrew or treated with saline for 1 week. c The growth curve of HCC827 xenograft tumors. d Quantitative RT-PCR analysis of miR-483-3p levels in regrown HCC827 xenograft tumors treated with gefitinib (resistant) for 6–8 weeks relative to saline-treated control tumors (sensitive). e–h Cell viability by CCK8 assay of indicated cells transiently transfected with miR-483-3p mimic, negative control (NC), miR-483-3p inhibitor, or inhibitor negative control (INC) as indicated in the presence or absence of indicated concentration of gefitinib for 72 h. i Agomir-483-3p mimic or agomir-NC was intratumorally injected into left or right regrown/resistant HCC827 xenograft tumor of each mouse, respectively, once every 4 days for a total four injections. Tumors were weighted and photographed (n = 5). For all panels: n = 5. ** P < 0.01; *** P < 0.001
