Fig. 2
miR-483-3p inhibits growth and promotes apoptosis in gefitinib-resistant cells. a–f HCC827GR and HCC827 were transiently transfected with miR-483-3p mimic (mimic), negative control (NC), miR-483-3p inhibitor (inhibitor), or inhibitor negative control (INC) as indicated. Cell proliferation was measured by EdU incorporation assay a, c (red) and cell viability assay b, d. Cell apoptosis was measured by flow cytometric apoptosis assay e and Western blot analysis of PARP and caspase-3 activation f. g, h Xenograft tumors were established using HCC827GR. Agomir-483-3p mimic or agomir-NC was intratumorally injected into left or right HCC827GR-xenograft tumor of each mouse, respectively, once every 4 days for a total four injections. Tumors were weighted and photographed g and then sectioned for H & E staining h, top panel, Ki67 immunostaining h, middle panel, and cleaved caspase-3 h, bottom panel (n = 5). i Agomir-483-3p mimic or agomir-NC was intratumorally injected into left or right regrown/resistant HCC827 xenograft tumor (resistant HCC827) of each mouse, respectively, once every 4 days for a total four injections. Tumors were sectioned for H&E staining h, top panel, Ki67 immunostaining h, middle panel, and cleaved caspase-3 h, bottom panel. For all panels: n = 5.** P < 0.01; *** P < 0.001
