Fig. 6 | Oncogene

Fig. 6

From: Epigenetic silencing of miR-483-3p promotes acquired gefitinib resistance and EMT in EGFR-mutant NSCLC by targeting integrin β3

Fig. 6

Integrin β3 overexpression rescues miR-483-3p-induced phenotypes. HCC827GR cells were co-transfected with miR-483-3p mimic (mimic) or negative control (NC) and LV-ITGB3 (ITGB3) or LV-GPF (LV) as indicated. a EdU incorporation assay (red, upper panel), immunostaining analysis of E-cadherin and vimentin (red, middle two panels), transwell invasion assay (bottom panel). b, c Quantification of EdU incorporation assay b and transwell invasion assay c of a. d Quantification of flow cytometric apoptosis assay. e Western blot analysis of integrin β3, E-cadherin, and vimentin expression. f Cell viability assay in the presence or absence of 1 μM gefitinib. For all panels: n = 5. *** P < 0.001

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