Fig. 3 | Oncogene

Fig. 3

From: DNA methyltransferase 3A isoform b contributes to repressing E-cadherin through cooperation of DNA methylation and H3K27/H3K9 methylation in EMT-related metastasis of gastric cancer

Fig. 3

DNMT3Ab induces the process of EMT. a Representative images of tumour cell morphology in DNMT3Aa- and DNMT3Ab-tranfected MKN45 and BGC-823 cells. b Western blot was used to show the expression of epithelial markers (E-cadherin and β-catenin) and mesenchymal markers (Vimentin and N-cadherin) in DNMT3Aa- or DNMT3Ab-transfected cells relative to that in the control cells. PCNA was used as a positive control. c Redistribution of actin filaments to filopodium-like structures in DNMT3Ab- transfected MKN45 cells, which were stained for F-actin. Double IF staining of E-cadherin and Vimentin was performed in DNMT3Ab-tranfected MKN45 cells. The cells were counterstained with DAPI to visualise the nuclei

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