Fig. 4

Blocking of the TIGIT-PVR/PVRL2 axis results in increased levels of Granzyme B secretion of immune cells. TF-1 (a–d) and MV4-11 (e, f) target cells were mixed with HD-PBMCs and incubated with blocking antibodies against PVR and PVRL2 or TIGIT in the presence or absence of the BiTE® antibody construct AMG 330. After 24 h, supernatants were harvested and human Granzyme B concentration was measured using ELISA. Results are depicted as the mean ± SD Granzyme B concentration of at least three independent experiments. For statistical analysis paired t-tests were performed (# p ≤ 0.05)