Fig. 4
From: LOX-catalyzed collagen stabilization is a proximal cause for intrinsic resistance to chemotherapy
Reduced supply is a direct effect of a Hif1α staining in non-vascularized spontaneous lung metastases (outlined) arising in mice implanted with 4T1 tumors. Yellow arrowheads: cells with nuclear Hif1α immunreactivity. b Quantification of tumor cells in non-vascularized 4T1 lung metastases with nuclear Hif1α staining (n = 4). c Histological and immunohistological analysis of MCTS generated from LOX/LOXL2 OE 4T1 cells. d Size distribution of 4T1 control and 4T1 LOX/LOX2 OE MCTS after 6 days of cultivation (n = 13). e Quantification of central necrosis in 4T1 control and 4T1 LOX/LOX2 OE MCTS (n = 8). f Quantification of Ki-6+ cells in 4T1 control and 4T1 LOX/LOX2 OE MCTS (n = 8). Error bars: ±SEM. SB = 100 µm. * indicates statistical significance vs. control, *P < 0.05, **P < 0.01
