Fig. 6

LOX(L) inhibition improves treatment efficacy in metastatic disease. a Treatment of established 4T1 BCAs with βAPN and/or DOX. b Weight of treated 4T1 BCAs 25 days after implantation. Combination of βAPN and DOX significantly reduced tumor size. c Quantification of metastatic nodules in the lungs of 4T1-implanted mice after a 14 days treatment course with βAPN and/or DOX. d Histological evaluation of metastases in the lungs of 4T1-implanted mice after a 14 days treatment course with βAPN and/or DOX. H&E and PSR staining of lung tissue. The metastases appear disintegrated after βAPN treatment. Collagen fibrils (arrows) are strongly reduced in size and quantity. e Cell density in metastases. Nuclei were quantified in H&E-stained sections (n = 6). f Quantification of PSR staining in metastases (n = 6). SB: 100 µm. Error bars: ±SEM. * indicates statistical significance vs. control, # indicates statistical significance of combination treatment group vs. both single treatment groups: *^,#P < 0.05, **^,##P < 0.01, ***P < 0.001