Fig. 1
From: Functional characterization of lysosomal interaction of Akt with VRK2
Interaction of VRK family of kinases with Akt in mammalian cells. a In coimmunoprecipitation experiments, FLAG-tagged VRK2 interacted with HA-tagged Akt in 293T cells. The expression of each construct is shown in the right-hand panel. b Among the three Akt isoforms, Akt1 and Akt2, but not Akt3, interacted with VRK2 in coimmunoprecipitation assays. The expression of each construct is shown in the right-hand panel. c The C terminus of Akt2 mediated the interaction with VRK2 in coimmunoprecipitation assays. The expression of each construct is shown in the right-hand panel. d WT VRK2A and WT VRK2B interacted with Akt through the N terminus of VRK2 in coimmunoprecipitation assays. A schematic representation of VRK2A and VRK2B is shown below. VRK2 has two alternatively spliced variants: VRK2A, containing 508 amino acid residues including the transmembrane domain, and VRK2B, containing 397 amino acid residues and lacking the transmembrane domain (Supplement 2a and b). Expression of each construct is shown in the right-hand panels
