Fig. 3
From: hMENA isoforms impact NSCLC patient outcome through fibronectin/β1 integrin axis
hMENA11a transfection significantly reduces ECM components in the secretome of breast tumor cells. a Heat map of proteins found to be differentially secreted in BT549 CNTR cells expressing hMENAΔv6 and in hMENA11a-expressing cell clones (#14 and #104) (left). Analysis was conducted on three biological replicates. T-tests and Benjamini and Hochberg’s FDR Control Algorithm was applied to the resulting large-scale data and FDR threshold used to generate the differentially expressed protein list. Fold change rank of proteins associated with ECM composition (right). b Heat map of proteins found to be differentially secreted across DAL CNTR cells and hMENA11a stable transfectants (left). Analysis was conducted on four biological replicates. Fold change rank of proteins associated with ECM composition also identified in BT549 cells (right). Western blot analysis of DAL CNTR and DAL/hMENA11a cells with the indicated Abs (inset). c Three networks identified by upstream regulator algorithm with predicted z-score action using the Ingenuity Pathway Analysis (IPA)
