Fig. 1
From: A SIRT1-centered circuitry regulates breast cancer stemness and metastasis
A SIRT1-KLF4-ALDH1A1-ALDH1A3 circuitry dictates age-related breast CSCs. a Functionally interacting network modules constructed from genes belonging to Age Human Genes list and stemness-associated genes were analyzed by Prism 5.0 tool (based on Pearson’s correlation coefficient) and visualized in Cytoscape. A graph that nodes have a power law distribution for their number of links, showing correlation between four factors, CSC markers and age-related genes in breast cancer cells (see Table S1). Bigger and darker colored nodes represent proteins with more links. Noted SIRT1 as the node at the tail end of the distribution on the graph. b Pathway enrichment analysis of four factors, CSC markers and their associated aging-related proteins by STRING database. Noted a significant enrichment of pathways that safeguard genome integrity, to which KLF4 and ALDH1A1/3 are the most correlated, followed by NANOG, then SOX2, and CD44 and OCT3/4 are the least. CD44 + CSCs are associated with low SIRT7 and share similar set of genes with OCT3/4. c Pearson correlation between SIRT1 and KLF4 mRNA levels in 52 breast cancer cell lines. R is the correlation coefficient
