Fig. 3 | Oncogene

Fig. 3

From: Loss of tuberous sclerosis complex 2 sensitizes tumors to nelfinavir−bortezomib therapy to intensify endoplasmic reticulum stress-induced cell death

Fig. 3

Nelfinavir enhances the cytotoxicity of bortezomib in human lung and colon cancer cell lines. a NCI-H460 lung cancer and HCT116 colon cancer cells were treated with either DMSO vehicle, 50 nM bortezomib (BTZ), 20 µM nelfinavir (NFV) alone, or NFV combined with BTZ over 24 h. Cells were then subjected to flow cytometry with DRAQ7 staining. DRAQ7 exclusion (below line) represents the viable cell population, while positive DRAQ7 staining (above line) indicates cell death. The number of DRAQ7-stained cells are graphed in (b) (n = 3). c With the addition of Etoposide (100 µM), cells were treated as in (a) and total protein levels of Caspase 8 (CASP8), Caspase 3 (CASP3), PARP, GADD34, CHOP and β-actin were measured by western blot analysis (n = 3)

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