Fig. 6 | Oncogene

Fig. 6

From: Loss of tuberous sclerosis complex 2 sensitizes tumors to nelfinavir−bortezomib therapy to intensify endoplasmic reticulum stress-induced cell death

Fig. 6

Nelfinavir and bortezomib upregulate early response genes for ER stress and cell death in Tsc2−/− MEFs, while prosurvival genes are downregulated. Tsc2+/+ and Tsc2−/− MEFs were treated with either DMSO vehicle or combined nelfinavir (20 µM) and bortezomib (50 nM) for 6 h before processing for RNA sequencing (n = 3). a Heatmap of a panel of ER stress-linked genes, a selection of which are highlighted graphically in (b). c Paired heatmaps from dual treated cells showing early response genes linked to cell survival and death which are highlighted in a volcano plot (d). e Tsc2+/+ and Tsc2−/− MEFs were treated with either DMSO vehicle or combined nelfinavir (20 µM) and bortezomib (50 nM) for 6, 16, and 24 h before extracting protein for western blot and probing for ATF4, CHOP, or β-actin (n = 3)

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