Fig. 3

Intercellular delivery of materials by PTDEs. a Mice were subcutaneously inoculated with GFP⁺ Huh-7 cells or RFP⁺ LM3 cells. Exosomes were isolated from the mice sera 2 weeks later. GFP and RFP were detected in the exosomes, respectively. b Naive Huh-7 cells were incubated with GFP⁺ Huh-7 cell-derived exosomes for 24 h. GFP was detected in the recipient cells by fluorescence. c Mice were intravenously injected with naive Huh-7 cells and GFP⁺ Huh-7-derived PTDEs successively. Mice were killed 2 h later, and GFP was detected in the lungs that contained intercepted Huh-7 cells. d HepG2 cells were incubated with PKH26-labeled exosomes derived from HepG2 cells for 2 h. Fluorescence was performed to identify nuclei (blue) and PKH26-labeled cell membrane (red). e, f Mice were treated as indicated. PKH26 (red)-labeled GFP⁺ Huh-7 cells were isolated from the mice circulation