Fig. 1
P2X7 mRNA is expressed in multiple cancer cell lines which do not show pore function. a Normalised ethidium influx in response to 0.5 mM BzATP stimulation in a panel of cancer cell lines. Mean of three independent experiments is shown. b Quantification of initial rate (5–40 min) of ethidium influx in 0.5 mM BzATP-treated cells above rate of increase in untreated cells. Mean and SEM from three independent experiments are shown. Two-tailed Student’s T-test was used to test whether agonist stimulation caused a significant increase in ethidium influx relative to untreated in each cell line. c Normalised ethidium influx in RPMI-8226 cells treated with 0.5 mM BzATP alone or in combination with P2X7 inhibitors A740003 or A438079 are shown. d Quantification of initial rate (5–30 min) of ethidium influx. Mean and SEM from three independent experiments are shown. One-way ANOVA with Dunnett’s post test was used to test significance. e P2RX7 mRNA quantification by qPCR in a panel of cancer cell lines. Mean and SEM from three independent experiments are shown. f Fura-2-loaded PC3 cells were pre-incubated with A740003 or AZ10606120, incubated in a fluorimeter cuvette in standard saline solution and challenged with 3 mM ATP. *P < 0.05
