Fig. 3
nfP2X7 is molecularly distinct from functional P2X7. a BIL03s and L4 binding relative to isotype control was measured by flow cytometry in a panel of cancer cell lines. Mean and SEM from at least three independent experiments are shown. b Western blotting of lysate from PC3 cells or PC3 cells overexpressing P2X7a. Representative images from three independent experiments are shown. c PC3 cells overexpressing P2X7a show increased L4 binding relative to untransfected PC3 but no change in BIL03s binding. Mean and SEM from three independent experiments are shown. Two-way ANOVA with Sidak’s multiple comparison test was used. d Ethidium influx into PC3 cells or PC3 cells overexpressing P2X7a in response to 0.5 mM BzATP or no agonist stimulation. Mean of three independent experiments shown. e Representation of the P2RX7 transcript and the target sequence location for siRNA. a, b, f The effect of P2RX7-targeted siRNA A and B on BIL03s and L4 binding in PC3 cells and PC3 cells overexpressing P2X7a was measured by flow cytometry. Mean and SEM from three independent experiments are shown. Two-way ANOVA with Dunnett’s post test was used to test significance. *P < 0.05, ***P < 0.001
