Fig. 2 | Oncogene

Fig. 2

From: Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration

Fig. 2

Expression of CXCR4 and AurA in human glioblastoma. a Western-blot analyses of CXCR4 and Tubulin in GBM1 and U87MG (n = 3). b FACS analyses of CXCR4 cell surface expression on GBM1 and U87MG cells (CT−: no antibody, CXCR4: mAb 12G5-PE) (n = 3). c Immunofluorescent stainings of CXCR4 (red), P-AurA (green), and Hoechst (blue) in GBM1 cells (n = 3). d Immunohistochemistry analysis of AurA (-DAB) in human non-cancerous (i.e., temporal lobe from epileptic patient) (n = 3) and GBM (n = 3) tissues (hematoxylin staining, 10×). The lower panel shows GBM tissue at 40× magnification. The graph shows the mean intensity of AurA staining by DAB in non-cancerous and GBM tissues quantified by Image J software. Scale bars = 100 μm. Graphs are mean values ± SD and are representative of three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001 (t-test corrected by post-tests if appropriate)

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