Fig. 5
MYEOV activates TGF-β signaling to trigger invasion-metastasis cascade. a Cignal 10-Pathway Reporter Array data obtained in A549 cells upon overexpression of MYEOV-cDNA, MYEOV-ATGmut, or MYEOV-miRmut (each bar represents the mean ± SD derived from three independent experiments, two-tailed Student’s t test. *P < 0.05). b WB analysis of p-SMAD3 in the indicated cells; β-actin was used as the loading control. c The number of invaded cells induced by MYEOV transcript following treatment with TGF-β inhibitor (LY2109761) (each bar represents the mean ± SD derived from three independent experiments, one-way ANOVA followed by Bonferroni’s multiple comparison test. *P < 0.05). d MYEOV transcript silencing-induced suppression of cell invasion was abrogated by transfection of CA-SMAD3 (each bar represents the mean ± SD derived from three independent experiments, two-tailed Student’s t test. *P < 0.05). e Representative bioluminescent images of tumor metastases in response to LY2109761 treatment. f Quantification of bioluminescent signal (each bar represents the mean ± SD derived from three independent experiments, one-way ANOVA followed by Bonferroni’s multiple comparison test. *P < 0.05). g Overall survival time of mice in indicated groups in the i.v. inoculation model (5 mice in each group)
