Fig. 2

Fstl1 depletion sensitizes GBM cells to TMZ. a Fstl1-depleted GBM cells were exposed to 200 μM TMZ for 48 h and the apoptosis was measured by flow cytometry. b Western blot analysis of MGMT, γ-H2AX, H2AX, caspase-3, and cleaved caspase-3 expression in Fstl1-depleted GBM cells or shCtrl cells in the presence of TMZ (200 μM, 48 h). c Colony formation assays were done with GBM cells infected with lenti-shFstl11–7 or lenti-shCtrl in the presence of TMZ (200 μM). d Representative pseudocolor bioluminescence images of intracranial xenografts bearing Fstl1-depleted P-GBM2 or shCtrl cells in the presence of TMZ on the days as indicated. Representative H&E staining for tumor cytostructure. e IHC analysis of MGMT, γ-H2AX, and cleaved caspase-3 expression in intracranial xenografts. f Survival curve of Fstl1-depleted P-GBM2 or shCtrl cells-derived intracranial xenografts treated with TMZ. g–i, p53-KD (knockdown) GBM cells transfected with Fstl1 or shFstl1-–7 were treated with TMZ (200 μM) for 48 h. Flow cytometry (g) and colony formation assays (i) were used to measure cells apoptosis and proliferation, western blot analysis of the indicated proteins expression (h). Student’s t tests were performed. Data are presented as mean ± SEM (**P < 0.01), scale bar = 100 μm