Fig. 3

YAP1-induced MALAT1 modulates the biological function of colon cancer cells in vitro and in vivo. a Wound-healing assay of HCT116 cells transfected with pEGFP-YAP1, pEGFP-YAP1 + MALAT1, or vector. b The effect of the YAP1–MALAT1 axis on migration was assessed in HCT116 cells after transfection with pEGFP-YAP1, pEGFP-YAP1 + MALAT1, or vector, by Transwell assay. c, d In vivo subcutaneous tumor xenografts stably transfected HCT116 cells in nude mice after injection of control, YAP1, RNAi control, or YAP1 + RNAi-MALAT1. After 28 days, the nude mice were sacrificed and the subcutaneous tumors were assessed macroscopically. Tumor weight reflected the proliferation ability of HCT116 cells in vivo. Four representative mice from each group are shown. e MALAT1 expression in various groups with in vivo subcutaneous tumors, as assessed by qRT-PCR. f Immunohistochemical detection of YAP1 protein levels in subcutaneous tumors from nude mice. Three independent experiments were performed. *p < 0.05, **p < 0.01 compared to the control group, by Student’s t-test