Fig. 4

Tamoxifen treatment inhibits the synthesis and secretion of the ECM proteins collagen-I and fibronectin (FN). a, c Representative images for immunofluorescence staining of HSCs, scale bar 50 µm. b, d Quantification of immunofluorescence staining for (a,c,e and f). b 16 control cells and 14 tamoxifen cells. d 20 control cells and 14 tamoxifen cells. e, f Representative images for immunofluorescence images of secreted collagen-I and FN. g qPCR levels of collagen-I and FN in HSCs, normalized to RPLP0 (60 S acidic ribosomal protein P0) and relative to control. h qPCR levels of collagen-I and FN in HSCs, normalized to RPLP0 and relative to 1 kPa, t-test for b, d and g; and Anova and Tukey’s test for (h). g, h three biological samples analysed in three different experiments. All histogram bars represent mean ± sem, *P < 0.05, **P < 0.01,***P < 0.001. Three experimental replicates in all cases