Fig. 5
Tamoxifen treatment mechanically inhibits the HIF-1A/LOX and HIF-1A/LOX-L2 axes. a, c and e Representative images for immunofluorescence staining of HSCs. b, d, f Quantification of immunofluorescence staining for (a, c and e). Scale bar is 50 µm for all panels. b 20 control cells and 20 tamoxifen cells. d 12 control cells and 14 tamoxifen cells. f 12 control cells and 14 tamoxifen cells. g qPCR levels of HIF-1A, LOX and LOX-L2 in HSCs, normalized to RPLP0 (60 S acidic ribosomal protein P0) and relative to control. h qPCR levels of HIF-1A, LOX and LOX-L2 in HSCs, normalized to RPLP0 and relative to 1 kPa. i qPCR levels of LOX and LOX-L2 in HSCs, normalized to RPLP0 and relative to control. Three biological samples analysed in three different experiments. All histogram bars represent mean ± sem, *P < 0.05, **P < 0.01,***P < 0.001. Three experimental replicates in all cases. t-test for (b, d and f), and Anova and Tukey’s test for (g, h and i)
