Fig. 2
Suppression of AGR2 by MG132 is independent of the induced ER stress and ROS. a Analysis of the effect of MG132 on proteins associated with ER stress by western blot. b A549 cells were incubated with ER stress inhibitor 4-PBA (10 mM) for 2 h prior to MG132 (5 μM) treatment of 24 h. p-eIF2a and AGR2 expression were determined by western blot analysis. c The level of AGR2 was detected with 10 μM salubrinal treatment for 24 h. d The expression changes of IRE1a and AGR2 in MG132-treated A549 cells. e Knockdown of ATF4 by siRNA. f The expression change of ATF4 in 5 μM MG132-treated A549 cells was estimated by western blotting analysis. g Knockdown of ATF4 by siRNA. h QRT-PCR and western blotting analysis of mRNA and protein levels of AGR2 in TG (2 μM)/TM (2 mg/ml)/MG132 (5 μM)-treated A549 cells. GAPDH served as a loading control. i A549 cells were treated with ER stress inducer TG/TM/MG132 treatment at indicated time
