Fig. 5
Autophagy stimulated by MG132 is required for the degradation of AGR2. a A549 cells were incubated with autophagy inhibitor 3-MA (5 mM) and CQ (10 μM) for 2 h prior to MG132 treatment. LC3B and AGR2 expression were determined by western blot analysis. b, c The expression changes of AGR2 in A549 cell, knockdown of b Atg5, and c Atg7 by siRNA for 48 h and then treated with MG132. d The level of AGR2 in Rapamycin (100 nM)-treated A549 cells. e A549 cells were treated with CHX and Rapamycin for 24 h. f The baseline expression of AGR2 and autophagy in normal bronchial epithelial cell line (HBE) and several lung cancer cell lines. GAPDH served as a loading control. g The expression changes of AGR2 with CQ treatment for 24 h. h, i The correlative analysis of AGR2 and p62/Atg5 in human lung cancer datasets (GSE27262) from GEO database was assessed by Pearson’s correlation test
