Fig. 4
SDF-1 secretion is elevated in the presence of cancer cells. a Circulating serum levels of SDF-1 (pg/ml) from either naïve or LLC/PyMT tumor bearing WT or dKO mice. Each dot represents one mouse. Data are presented as mean ± SD, One-way ANOVA followed by Tukey’s post-tests, n = 4, *—difference compared to WT **p < 0.01; ***p < 0.001; #—difference compared to naïve mice; †—difference between tumor type. Representative image of at least three experiments. b WT or dKO MEFs (7.5 × 105) were co-cultured with LLC cells (2.5 × 105) and as a control: LLC, WT MEFs and dKO MEFs (1 × 106) were cultured in serum-free (SF) medium for 24 h. The levels of SDF-1 secreted to the medium were measured (pg/ml). Each dot represents one plate. Data are presented as mean ± SD, One-way ANOVA followed by Tukey’s post-tests, n = 4; * - difference between genotypes- **p < 0.01; ***p < 0.001; #—difference between PyMT and LLCs; †—difference between treatments. Representative image of at least three experiments. c WT and dKO MEFs were cultured in the presence of 10% FBS (control) or LLC conditioned medium (CM). After 24 h, the medium was replaced with SF medium, which was then collected after 24 h. The levels of SDF-1 secreted to the medium was measured and analyzed as described in (b). Each dot represents one plate. Data are presented as mean ± SD, One-way ANOVA followed by Tukey’s post-tests n = 12,12,8,8; *—difference between genotypes ***p < 0.001; #—difference compared to WT MEFs- Control. Representative image of two experiments. In all graphs, levels of SDF-1 were assessed by ELISA
